According to legal documents obtained by CNN on Monday, she filed last week in Williamson County, Tennessee, citing “Irreconcilable differences.”
The filing states that the couple, who married in 1993, have not lived together in more than two years.
The pair are the parents of five adult children: sons Trace (33) and Braison (27) and daughters Brandi (34), Miley (29) and Noah (22).
Billy Ray Cyrus, 60, filed for divorce in 2010, and Tish Cyrus, 54, filed in 2013. Both times the couple reconciled.
A representative for Billy Ray Cyrus declined to comment about the latest divorce filing.
“We both went into couples therapy something we haven’t done in 22 years of being together, and its brought us closer together and really opened up our communication in amazing ways,” the statement said at the time. “Tish also said marriage can be really hard especially after 22 years of being in entertainment. We’ve had rough times but we both realized we didn’t want to be another statistic and wanted to make it work.”